BioJava:CookBook:AAPROP:commandprompt
How can I compute physico-chemical properties using Command Prompt?
1) Download
biojava3-aa-prop-3.0.2-SNAPSHOT-jar-with-dependencies.jar
and rename it to AAProperties.jar
2) Study the Manual section below
Note: Test.fasta is available if you would need
sample fasta sequences.
Manual
NAME
An executable to generate physico-chemical properties of protein sequences.
EXAMPLES
java -jar AAProperties.jar -i test.fasta -a
Generates all possible properties.
java -jar AAProperties.jar -i test.fasta -1 -3 -7
Generates only molecular weight, extinction coefficient and isoelectric point.
java -jar AAProperties.jar -i test.fasta -0 A -0 N -1
Generates composition of two specific amino acid symbol and molecular weight.
OPTIONS
Required
-i location of input FASTA file
Optional
-o location of output file [standard output (default)]
-f output format [csv (default) or tsv]
-x location of Amino Acid Composition XML file for defining amino acid composition
-y location of Element Mass XML file for defining mass of elements
-d number of decimals (int) [4 (default)]
Provide at least one of them
-a compute properties of option 1-9
-1 compute molecular weight
-2 compute absorbance
-3 compute extinction coefficient
-4 compute instability index
-5 compute apliphatic index
-6 compute average hydropathy value
-7 compute isoelectric point
-8 compute net charge at pH 7
-9 compute composition of 20 standard amino acid (A, R, N, D, C, E, Q, G, H, I, L, K, M, F, P, S, T, W, Y, V)
-0 compute composition of specific amino acid symbol