001/* 002 * BioJava development code 003 * 004 * This code may be freely distributed and modified under the 005 * terms of the GNU Lesser General Public Licence. This should 006 * be distributed with the code. If you do not have a copy, 007 * see: 008 * 009 * http://www.gnu.org/copyleft/lesser.html 010 * 011 * Copyright for this code is held jointly by the individual 012 * authors. These should be listed in @author doc comments. 013 * 014 * For more information on the BioJava project and its aims, 015 * or to join the biojava-l mailing list, visit the home page 016 * at: 017 * 018 * http://www.biojava.org/ 019 * 020 */ 021 022package org.biojava.nbio.genome.util; 023 024import org.biojava.nbio.core.sequence.DNASequence; 025import org.biojava.nbio.core.sequence.io.FastaReaderHelper; 026import org.biojava.nbio.core.sequence.io.FastaWriterHelper; 027import org.slf4j.Logger; 028import org.slf4j.LoggerFactory; 029 030import java.io.File; 031import java.util.ArrayList; 032import java.util.LinkedHashMap; 033import java.util.Map; 034 035 036/** 037 * Utility to write each Fasta entry to a unique file 038 * @author Scooter Willis 039 */ 040public class SplitFasta { 041 042 private static final Logger logger = LoggerFactory.getLogger(SplitFasta.class); 043 044 public void processNucleotides(File fastaFileName,String uniqueid, File outputDirectory ) throws Exception{ 045 if(!outputDirectory.exists()) 046 outputDirectory.mkdirs(); 047 048 Map<String,DNASequence> dnaSequenceHashMap = FastaReaderHelper.readFastaDNASequence(fastaFileName); 049 for(DNASequence dnaSequence : dnaSequenceHashMap.values()){ 050 String fileName = outputDirectory.getAbsolutePath() + File.separatorChar; 051 if(uniqueid.length() > 0){ 052 fileName = fileName + dnaSequence.getAccession().getID() + ".fna"; 053 }else{ 054 fileName = fileName + uniqueid + dnaSequence.getAccession().getID() + ".fna"; 055 } 056 ArrayList<DNASequence> dnaList = new ArrayList<>(); 057 dnaList.add(dnaSequence); 058 FastaWriterHelper.writeNucleotideSequence(new File(fileName), dnaList); 059 } 060 061 } 062 063 public static void main( String[] args ){ 064 try{ 065 SplitFasta splitFasta = new SplitFasta(); 066 splitFasta.processNucleotides(new File("/Users/Scooter/scripps/dyadic/analysis/454Scaffolds/454Scaffolds.fna"), "", new File("/Users/Scooter/scripps/dyadic/analysis/454Scaffolds/individual")); 067 }catch(Exception e){ 068 logger.error("Exception: ", e); 069 } 070 } 071 072}