001/* 002 * BioJava development code 003 * 004 * This code may be freely distributed and modified under the 005 * terms of the GNU Lesser General Public Licence. This should 006 * be distributed with the code. If you do not have a copy, 007 * see: 008 * 009 * http://www.gnu.org/copyleft/lesser.html 010 * 011 * Copyright for this code is held jointly by the individual 012 * authors. These should be listed in @author doc comments. 013 * 014 * For more information on the BioJava project and its aims, 015 * or to join the biojava-l mailing list, visit the home page 016 * at: 017 * 018 * http://www.biojava.org/ 019 * 020 */ 021 022package org.biojava.bio.molbio; 023 024import org.biojava.bio.seq.StrandedFeature; 025 026/** 027 * <code>RestrictionSite</code> represents the recognition site of a 028 * restriction enzyme. 029 * 030 * @author Keith James 031 * @since 1.3 032 */ 033public interface RestrictionSite extends StrandedFeature 034{ 035 /** 036 * <code>getPosition</code> returns the common, forward strand cut 037 * site. Note that some enzymes cut in more than one 038 * position. Such supplementary sites may be calculated by 039 * retrieving the <code>RestrictionEnzyme</code> instance and 040 * using its methods to calculate the position. 041 * 042 * @return an <code>int</code> indicating the base immediately 043 * before the cleavage site on the forward strand. 044 */ 045 public int getPosition(); 046 047 /** 048 * <code>getEnzyme</code> returns the enzyme which cuts at this 049 * site. A sequence which is the target for several different 050 * enzymes is expected to have a corresponding 051 * <code>RestrictionSite</code> feature for each. 052 * 053 * @return a <code>RestrictionEnzyme</code>. 054 */ 055 public RestrictionEnzyme getEnzyme(); 056 057 /** 058 * <code>Template</code> for construction of 059 * <code>RestrictionSite</code>s. 060 */ 061 public static class Template extends StrandedFeature.Template 062 { 063 /** 064 * <code>enzyme</code> <code>RestrictionEnzyme</code> field. 065 */ 066 public RestrictionEnzyme enzyme; 067 } 068}